FISH-Flow
Fluorescence in situ Hybridisation
We have coupled flow cytometry with Fluorescent in situ Hybridisation (FISH) to permit rapid assessment of the important functional bacterial groups within an intestinal microbiota.
Unlike sequencing, this technology measures cell numbers, as opposed to nucleic acid transcripts, and therefore is fully quantitative. We analyse the following major bacterial groups which have been implicated in host health and disease:
- All Eukaryotes
- Bifidobacterium genus
- Lactobacillus-Enterococcus group
- Bacteroides
- Clostridium coccoides group
- Roseburia
- Atopobium cluster
- Clostridia cluster IX
- Faecalibacterium prausnitzii subgroup
- Desulphovibrionaceae & some Geobactaraceae
- Clostridium histolyticum group
Dynamics in Bacteria Populations
Such observational changes can be linked to functional/biological effects, for example, metabolite production. We measure specific metabolite production following intervention with the test substrate using High Pressure Liquid Chromatography (HPLC) and Gas Chromatography (GC). These include, but are not limited to,
- Organic acids (acetate, butyrate, propionate, lactate, formate),
- Branched chain fatty acids (valerate, iso-valerate, iso-butyrate)
- Phenolic compounds (p-cresol, indole, phenol and skatole, amines, ammonia).
Short Chain Fatty Acid Analysis
We offer basic statistical analysis of the data generated using univariate linear regression and least-significant differences methodologies.
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